Journal: Aesthetic Surgery Journal. Open Forum

Article Title: The Effect of Hybrosome (Umbilical Cord Blood Exosome–Liposome Hybrid Vesicles) on Human Dermal Cells In Vitro

doi: 10.1093/asjof/ojad039

Figure Lengend Snippet: Confocal scanning microscope (20 μm scale bar, 20× magnification) image of the immunocytochemistry analysis of HDF cells to evaluate collagenase Type I and elastin expressions. Untreated control cells stain relatively weakly when stained with antibodies against collagen Type I and elastin than cells that have been treated with the hybrosome. DAPI-stained nuclei appear blue. Three independent experiments were repeated for immunocytochemistry studies and only 1 result image added for each group. DAPI, diamidino-2-phenylindole; HDF, human dermal fibroblast cell line.

Article Snippet: For nuclei examination, cells were stained with 0.1% DAPI solution (4′,6-di-di-amidino-2-phenylindole; AppliChem, Darmstadt, Germany) for 20 min at 4°C and washed with PBS-T.

Techniques: Microscopy, Immunocytochemistry, Control, Staining

Journal: Radiation Oncology (London, England)

Article Title: Effects of osteopontin inhibition on radiosensitivity of MDA-MB-231 breast cancer cells

doi: 10.1186/1748-717X-5-82

Figure Lengend Snippet: PARP protein levels and apoptosis rate of either non-irradiated or irradiated cells after siRNA transfection . A . Western blot analysis of PARP with rabbit anti-human cleaved PARP (Asp214) antibody in MDA-MB-321 cells 24 h and 72 h after transfection. The cells were untreated (UT), transfected with 100 nM of either nonsense siRNA (non) or target-specific siRNAs to knockdown OPN (Mix and OpnS). The Western blot shows one representative result out of three independent experiments. Actin served as an internal loading control. B . The morphology of DAPI stained cell nuclei was analyzed to quantify the apoptosis rate of MDA-MB-231 cells 72 h after transfection. The diagram shows the apoptosis rate of the cells as a function of treatment and irradiation. A fluorescence microscope was used and 500 cells in several fields of view were counted for each experiment. Data represent the average values (± SD) of three independent experiments (* p < 0.05, ** p < 0.001).

Article Snippet: The cells were fixed with 80% ethanol (Merck, Darmstadt, Germany) and centrifuged on microscope slides at 1000 g for 5 min. After staining with DAPI solution (4,6-diamidino-2-phenylindole dihydrochloride) (Serva, Heidelberg, Germany) and washing with PBS, the cells were covered with ProLong ® Gold antifade reagent (Invitrogen).

Techniques: Irradiation, Transfection, Western Blot, Knockdown, Control, Staining, Fluorescence, Microscopy